►曾经认为AC-13核型对系统性红斑狼疮具有高度特异性,但这种特异性存在争议(69,70)
►如果临床上疑似系统性红斑狼疮,建议进行PCNA抗体检测;几种常规的ENA谱中包含此抗原(69)
►抗原特异性免疫检测的最新研究表明,与系统性硬化症,自身免疫性肌病,类风湿关节炎,丙型肝炎和其他疾病也有临床相关性(70-73)
简体中文 | English | |
同义词 | 无 | none |
描述 | 呈现形态多样的颗粒状荧光,且颗粒大小和亮度不一;在G1期,部分间期细胞阴性,但在S期则有较强的荧光。此外,在S晚期和G2早期的细胞可呈现零星颗粒荧光,核仁偶有荧光,分裂期细胞阴性。 |
Pleomorphic speckled nucleoplasmic staining, with variability in size and brightness of the speckles. In interphase, some cells are negative (G1 phase), some are intensely stained (S-phase) and some present rare and scattered speckles with occasional nucleolar staining (late S and early G2 phases). Mitotic cells are not stained. |
抗原相关性 | 增殖细胞核抗原 | PCNA |
►曾经认为AC-13核型对系统性红斑狼疮具有高度特异性,但这种特异性存在争议(69,70)
►如果临床上疑似系统性红斑狼疮,建议进行PCNA抗体检测;几种常规的ENA谱中包含此抗原(69)
►抗原特异性免疫检测的最新研究表明,与系统性硬化症,自身免疫性肌病,类风湿关节炎,丙型肝炎和其他疾病也有临床相关性(70-73)
► The AC-13 pattern has formerly been considered highly specific for SLE, but this specificity is debated (69, 70)
► If SLE is clinically suspected, it is recommended to perform a follow-up test for anti-PCNA antibodies; the antigen is included in several routine ENA profiles (69)
► Recent studies with antigen-specific immunoassays show clinical associations also with SSc, AIM, RA, HCV, and other conditions (70–73)
69. Miyachi K, Fritzler MJ, Tan EM. Autoantibody to a nuclear antigen in proliferating cells. J Immunol 1978;121:2228–34.
70. Vermeersch P, De Beeck KO, Lauwerys BR, et al. Antinuclear antibodies directed against proliferating cell nuclear antigen are not specifically associated with systemic lupus erythematosus. Ann Rheum Dis 2009;68:1791–3.
71. Mahler M, Burlingame RW, Wu J, et al. Serological and clinical associations of anti-PCNA antibodies in systemic lupus erythematosus detected by a novel chemiluminescence assay. Arthritis Rheum 2011;63.
72. Mahler M, Miyachi K, Peebles C, et al. The clinical significance of autoantibodies to the proliferating cell nuclear antigen (PCNA). Autoimmun Rev 2012;11:771–5.
73. Hsu TC, Tsay GJ, Chen TY, et al. Anti-PCNA autoantibodies preferentially recognize C-terminal of PCNA in patients with chronic hepatitis B virus infection. Clin Exp Immunol 2006;144:110–6.
69. Miyachi K, Fritzler MJ, Tan EM. Autoantibody to a nuclear antigen in proliferating cells. J Immunol 1978;121:2228–34.
70. Vermeersch P, De Beeck KO, Lauwerys BR, et al. Antinuclear antibodies directed against proliferating cell nuclear antigen are not specifically associated with systemic lupus erythematosus. Ann Rheum Dis 2009;68:1791–3.
71. Mahler M, Burlingame RW, Wu J, et al. Serological and clinical associations of anti-PCNA antibodies in systemic lupus erythematosus detected by a novel chemiluminescence assay. Arthritis Rheum 2011;63.
72. Mahler M, Miyachi K, Peebles C, et al. The clinical significance of autoantibodies to the proliferating cell nuclear antigen (PCNA). Autoimmun Rev 2012;11:771–5.
73. Hsu TC, Tsay GJ, Chen TY, et al. Anti-PCNA autoantibodies preferentially recognize C-terminal of PCNA in patients with chronic hepatitis B virus infection. Clin Exp Immunol 2006;144:110–6.
►在推导AC-13核型与经典35 kDa PCNA(DNA聚合酶δ辅助蛋白的延伸因子)的抗体之间相关性的主要挑战是:已知“ PCNA”是一种大分子复合物,其中存在除“经典”35kDa PCNA之外的其他靶抗原。此外,许多其他明显不相关的靶抗原也可以通过间接免疫荧光法-HEp-2基质产生类似AC-13的核型(27)
27. Takeuchi K, Kaneda K, Kawakami I, et al. Autoantibodies recognizing proteins copurified with PCNA in patients with connective tissue diseases. Mol Biol Rep 1996;23:243‐6.