►AC-11核型很少在常规的自身抗体检测中发现，在自身免疫性细胞减少症、自身免疫性肝病、带状硬皮病、抗磷脂综合征和系统性自身免疫性风湿疾病中都有描述；目前关于临床相关性的信息主要基于病例报告和小群体研究(58-60)
►被识别的抗原包括lamins (A、B、C)和LAP-2;目前还没有针对这些自身抗体的特异性免疫检测商品化试剂盒（58-60）
| 简体中文 | ||
| 同义词 | 核边缘，核膜，膜状 | nuclear rim, nuclear membrane, membranous |
| 描述 | 细胞核呈现均匀荧光，核周增强；分裂中期和后期细胞的染色体阴性；临近细胞相触部位荧光增强。例如抗板层素B抗体。 |
Homogeneous staining of the nucleus with greater intensity at its outer rim and no staining at the metaphase and anaphase chromatin plates. There is a peculiar accentuation of the fluorescence at the points where adjacent cells touch each other. e.g. anti-lamin B. |
| 抗原相关性 | 板层素A，B，C，或板层素相关蛋白 | lamins A,B,C, or lamin-associated proteins |
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► The AC-11 pattern is infrequently found in routine autoantibody testing and has been described in autoimmune-cytopenias, autoimmune liver diseases, linear scleroderma, APS, and SARD; current information on clinical associations is based mainly on case reports and small cohorts (58–60)
► Antigens recognized include lamins (A, B, C) and LAP-2; specific immunoassays for these autoantibodies are currently not commercially available (58–60)
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一级信息参考文献First level information references
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58. Coppo P, Clauvel JP, Bengoufa D, et al. Autoimmune cytopenias associated with autoantibodies to nuclear envelope polypeptides. Am J Hematol 2004;77:241–9.
59. Konstantinov K, Foisner R, Byrd D, et al. Integral membrane proteins associated with the nuclear lamina are novel autoimmune antigens of the nuclear envelope. Clin Immunol Immunopathol 1995;74:89–99.
60. Reeves WH, Chaudhary N, Salerno A, et al. Lamin B autoantibodies in sera of certain patients with systemic lupus erythematosus. J Exp Med 1987;165:750–62.
58. Coppo P, Clauvel JP, Bengoufa D, et al. Autoimmune cytopenias associated with autoantibodies to nuclear envelope polypeptides. Am J Hematol 2004;77:241–9.
59. Konstantinov K, Foisner R, Byrd D, et al. Integral membrane proteins associated with the nuclear lamina are novel autoimmune antigens of the nuclear envelope. Clin Immunol Immunopathol 1995;74:89–99.
60. Reeves WH, Chaudhary N, Salerno A, et al. Lamin B autoantibodies in sera of certain patients with systemic lupus erythematosus. J Exp Med 1987;165:750–6
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二级信息Second level information
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二级信息参考文献Second level information references
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FAQ
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细胞核周阳性但中心阴性？有时可观察到细胞核的外周有类似阳性的染色，而细胞核中心是阴性的，这种现象的原因是什么？
核膜型（AC-11和AC-12）会在分裂间期细胞的细胞核周围边界处呈现独特染色，同时由于分裂期核膜破裂，分裂期细胞中没有染色。有时分裂间期的细胞核上可能有微弱染色，为核膜染色叠加所致。除AC-11和AC-12代表的靶抗原外，尚无其他已知的仅位于HEp-2细胞核外周的自身抗原。偶尔有某些样本会呈现核均质染色（AC-1），在核膜附近略有增强，根据我们的经验，使用其他品牌或同品牌不同批次的HEp-2玻片再次检测不会观察到该现象。因此这种特定的荧光分布可能是由细胞培养及固定条件产生的伪影。另外也可能是在IFA操作的孵育期间，细胞基质意外干燥引起的伪影。日期：2019年04月05日