► AC-27核型对任何疾病的阳性预测值均较低(116)

►在常规血清学诊断中非常少见(117)

►所识别的抗原包括 CENP-E、CENP-F、TD60、MSA36、KIF-14、MKLP-1、MPP1/KIF20B和 INCENP；这些自身抗体的特异性免疫检测试剂目前尚未商品化 (116, 118, 119)

► The AC-27 pattern has low positive predictive value for any disease (116)

► Found very infrequently in a routine serology diagnostic setting (117)

► Antigens recognized include, among other, CENP-E, CENP-F, TD60, MSA36, KIF-14, MKLP-1, MPP1/KIF20B, and INCENP; specific immunoassays for these autoantibodies are currently not commercially available (116, 118, 119)

116. Rattner JB, Mack GJ, Fritzler MJ. Autoantibodies to components of the mitotic apparatus. Mol Biol Rep 1998;25:143–55.

117. Vermeersch P, Bossuyt X. Prevalence and clinical significance of rare antinuclear antibody patterns. Autoimmun Rev 2013;12:998–1003.

118. Humbel RLConrad K, ed. Autoantibodies to mitotic cells. In Dresden autoantibody symposium. Dresden Germany: Pabst Science, 2013: 327–39.

119. Rodriguez-Bayona B, Ruchaud S, Rodriguez C, et al. Autoantibodies against the chromosomal passenger protein INCENP found in a patient with Graham Little-Piccardi-Lassueur syndrome. J Autoimmune Dis 2007;4.

116. Rattner JB, Mack GJ, Fritzler MJ. Autoantibodies to components of the mitotic apparatus. Mol Biol Rep 1998;25:143–55.

117. Vermeersch P, Bossuyt X. Prevalence and clinical significance of rare antinuclear antibody patterns. Autoimmun Rev 2013;12:998–1003.

118. Humbel RLConrad K, ed. Autoantibodies to mitotic cells. In Dresden autoantibody symposium. Dresden Germany: Pabst Science, 2013: 327–39.

119. Rodriguez-Bayona B, Ruchaud S, Rodriguez C, et al. Autoantibodies against the chromosomal passenger protein INCENP found in a patient with Graham Little-Piccardi-Lassueur syndrome. J Autoimmune Dis 2007;4.

►在系统性硬化症、雷诺现象和恶性肿瘤的患者中偶有检出(10-12)

►对 CENP-E、CENP-F、TD60、MSA36、KIF-14和MKLP-1的自身抗体在系统性硬化症 (局限型和弥漫型)、系统性红斑狼疮和恶性肿瘤患者中均有报道 (13,14)

►INCENP自身抗体曾在一例假性斑秃-毛周角化综合征患者中被检出 (15)

►MPP1/KIF20B (M期磷蛋白1) 自身抗体曾在特发性共济失调、其他神经综合征和阵发性夜间血红蛋白尿的患者中被检出 (16,17)

注：大多数报道都描述了自身抗体直接与特异性抗原结合(即抗原特异性免疫分析)，实际并未显示与AC-27模式相关性；这些自身抗体的特异性免疫检测试剂目前尚未商品化。

► Incidentally described in patients with SSc, Raynaud’s phenomenon, and malignancy (10-12)

► Autoantibodies to CENP-E, CENP-F, TD60, MSA36, KIF-14, and MKLP-1 have been described in patients with SSc (limited and diffuse), SLE, and malignancies (13, 14)

► Autoantibodies to INCENP have been described in a single patient with Graham-Little-Piccardi-Lasseur Syndrome (15)

► Autoantibodies to MPP1 /KIF20B (M-phase phosphoprotein 1) have been described in patients with idiopathic ataxia, other neurological syndromes and paroxysmal nocturnal haemoglobinuria (16, 17)

Notes: Most reports describe autoantibodies directly binding to specific antigens (i.e., antigen-specific immunoassays) and do not actually show correlations with the AC-27 pattern as such; specific immunoassays for these autoantibodies are currently not commercially available.

10. Fritzler MJ, Ayer LM, Gohill J, et al. An antigen in metaphase chromatin and the midbody of mammalian cells binds to scleroderma sera. J Rheumatol 1987;14:291-4.

11. Tausche AK, Conrad K, Seidel W, et al. Anti-midbody antibodies as a possible predictive factor for a special limited or abortive form of systemic sclerosis? Ann Rheum Dis 2005;64:1237-8.

12. Vermeersch P, Bossuyt X. Prevalence and clinical significance of rare antinuclear antibody patterns. Autoimmun Rev 2013;12:998-1003.

13. Rattner JB, Mack GJ, Fritzler MJ. Autoantibodies to components of the mitotic apparatus. Mol Biol Rep 1998;25:143-55.

14. Humbel RL. Autoantibodies to mitotic cells. in Dresden Autoantibody Symposium (eds. Conrad, K., et al.) 327-39 (Pabst Science, Dresden, Germany, 2013).

15. Rodriguez-Bayona B, Ruchaud S, Rodriguez C, et al. Autoantibodies against the chromosomal passenger protein INCENP found in a patient with Graham Little-Piccardi-Lassueur syndrome. J Autoimmune Dis 2007;4:1.

16. Fritzler MJ, Kerfoot SM, Feasby TE, et al. Autoantibodies from patients with idiopathic ataxia bind to M-phase phosphoprotein 1 (MPP-1). J Invest Med 2000;48:28-39.

17. Alahmad A, Preuss KD, Schenk J, et al. Desmoplakin and KIF20B as target antigens in patients with paroxysmal nocturnal haemoglobinuria. Br J Haematol 2010;151:273-80.

10. Fritzler MJ, Ayer LM, Gohill J, et al. An antigen in metaphase chromatin and the midbody of mammalian cells binds to scleroderma sera. J Rheumatol 1987;14:291-4.

11. Tausche AK, Conrad K, Seidel W, et al. Anti-midbody antibodies as a possible predictive factor for a special limited or abortive form of systemic sclerosis? Ann Rheum Dis 2005;64:1237-8.

12. Vermeersch P, Bossuyt X. Prevalence and clinical significance of rare antinuclear antibody patterns. Autoimmun Rev 2013;12:998-1003.

13. Rattner JB, Mack GJ, Fritzler MJ. Autoantibodies to components of the mitotic apparatus. Mol Biol Rep 1998;25:143-55.

14. Humbel RL. Autoantibodies to mitotic cells. in Dresden Autoantibody Symposium (eds. Conrad, K., et al.) 327-39 (Pabst Science, Dresden, Germany, 2013).

15. Rodriguez-Bayona B, Ruchaud S, Rodriguez C, et al. Autoantibodies against the chromosomal passenger protein INCENP found in a patient with Graham Little-Piccardi-Lassueur syndrome. J Autoimmune Dis 2007;4:1.

16. Fritzler MJ, Kerfoot SM, Feasby TE, et al. Autoantibodies from patients with idiopathic ataxia bind to M-phase phosphoprotein 1 (MPP-1). J Invest Med 2000;48:28-39.

17. Alahmad A, Preuss KD, Schenk J, et al. Desmoplakin and KIF20B as target antigens in patients with paroxysmal nocturnal haemoglobinuria. Br J Haematol 2010;151:273-80.